Welcome to ExosomesTalk, a new initiative for scientists in the exciting field of exosome research.

Join the conversations by responding to questions posted by your fellow scientists, and post your own questions.

ExosomesTalk is curated by scientists at Life Technologies. , with all content focused on your research needs. Our goal is to help you achieve faster breakthroughs by building products and services tailored to your needs.

Exosome purification from hemolyzed blood sample

Hi,

I have a sample of blood collected in a serum provette which was placed at -80°C without first centrifuge (to let the gel separator divide serum from coagulated blood).

Now I defrost the sample in order to separate the serum but obviously the blood hemolyzed.

Would it affect the isolation of exosomes? (I use Exoquik kit)

Best Regards

Bonetti Michele
asked Mar 9, 2015 in ExosomesTalk by MBonetti
edited Mar 9, 2015 by MBonetti

1 Answer

Hi,

We have not tested this and I am not aware of others that have tried this. I can only recommend to try.

kind regards

ketil
answered Jun 10, 2015 by ketil.pedersen
...