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How to best isolate extracellular vesicles (exosomes and microparticles) derived from endothelial cells in routine serum samples from patients?

asked Oct 8, 2013 in ExosomesTalk by Anita

1 Answer

with the Total exosome isolation (from serum) reagent you can recover the total population of exosomes. If you want to specifically isolate the certain population after that - the best approach would be to use affinity capture, eg magnetic beads with antibodies (or aptamers) specific to certain protein markers on the surface of exosomes. The tricky part is - we dont have a good understanding of exosomal markers that would indicate cell of origin.
answered Oct 8, 2013 by Vlassosv
The Total exosome Isolation kit (from serum) isolate vesicles like exosomes (50-100nm)? Anybody know the mean vesicle size isolated? Are also larger vesicles (microparticles) isolated by this kit? The vesicle sizes between 100-500 nm (small microparticles) are also of interest to me because  they are below the detection limit for my flow, as well as from 500-1200 nm (detectable by flow cytometry after isolation by centrifugation). Would be really nice if there were isolation kits for the whole range of extracellular microvesicles (50-1200 nm).
when we analyze the recovered vesicles on Nanosight- we see some particles larger that exosomes (>150 nm), but they are present in small amounts. It also depends on the body fluids- some have more, some have less. So you can test our Total reagent for your project, but also explore eg filtration devices- to capture the population of nano/microvesicles 50-1200 nm in size.