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Does anyone characterize exosome marker by RT-PCR

I want to know if it possible for charaterization exosome by reverstranscriptase polymerase chain reaction. I used exosome marker such as CD9, CD81, annexinA1, annexinA2 and clusterin. I used GAPDH as a housekeeping gene.
If it possible to find that markers

or I should use westren blot.
asked Mar 26, 2015 in ExosomesTalk by BiggieTanapong

1 Answer

Hi, 

in terms of RT-PCR/WB you may check out our publication in Clinical Therapeutics.  This publication can be downloaded at my ResearchGate profile: https://www.researchgate.net/profile/Ketil_Pedersen?ev=hdr_xprf

hope this will be of help.

 

kind regards

ketil

answered Mar 30, 2015 by ketil.pedersen
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