Welcome to ExosomesTalk, a new initiative for scientists in the exciting field of exosome research.

Join the conversations by responding to questions posted by your fellow scientists, and post your own questions.

ExosomesTalk is curated by scientists at Life Technologies. , with all content focused on your research needs. Our goal is to help you achieve faster breakthroughs by building products and services tailored to your needs.

Is there a difference in the size distribution of exosomes isolated from different kind of samples ?

Hi,

Based on your experience, is there a known difference in the size range of exosomes isolated from cell culture supernatant and from serum/plasma ?
Thanks!
asked Aug 7, 2015 in ExosomesTalk by GiBro

1 Answer

Hi,  thanks for the question.
thats difficult to answer. so this will only be speculations. As far as I have observed using electron microscopy the size distribution seems to be narrow. If exosomes are produced in the endocytic compartment forming the multivesicular bodies and using the same invagination machinery I would guess this would produce vesicles of similar size - equivalent to the formation of clathrin coated pits and clathrin coated vesicles. but going forward in this field will for sure tell us in greater detail the mechanisms involved in the MVB formation and exosome release.
kind regards

ketil
answered Aug 26, 2015 by ketil.pedersen
...