We have used Dynabeads coated with anti-CD63, CD81 or CD9 to pull out vesicles from pre-enriched preparations using Total Exosome Isolation kit and prepare them for electron microscopy. The size distribution of the vesicle located on the surface of the magnetic beads look very similar and small in size (<100nm) and also similar to exosomes prepared by ultracentrifugation.
For exosome workflow see:
- Oksvold et al Clinical Therapeutics 2014 Vol 36 no 6
- Oksvold et al Methods Mol Biol 2015, 1218, 465-81.
- Zeringer et al World J Methodol 03/2013; 26(3(1))