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The exosomes isolated by The Total Exosome Isolation (from serum) reagent were very difficult to resuspend,how to solve the probleme?

The exosomes isolated by The Total Exosome Isolation (from serum) reagent were very difficult to resuspend with Trizol,RIPA,pbs or ddH20,how to solve the probleme?
asked Feb 25, 2014 in ExosomesTalk by sophia_y12y

2 Answers

 
Best answer
we routinely resuspend the exosomes in PBS. The trick is to use the lower volume of serum- eg 100 uL. In this case the obtained exosome pellet is small, easy to resuspend, experiment is cheaper (as you use less reagent), but you still recover plenty of exosomes for any type of downstream analysis. With larger volumes (eg 1 mL serum or more) the pellet is bigger and sometimes tough to resuspend. in this case add PBS or other buffer and let it sit eg 1 h at RT. then pipet or gently vortex
answered Feb 27, 2014 by Vlassosv
selected Feb 28, 2014 by sophia_y12y
thank you for your answer.

i  do the same experiment  with you ,if you want to resuspend the exosomes ,just use Nuclease-Free Water,then use tip to blow. i hope this suggestion is helpful for you!!!

answered Feb 27, 2014 by duizhang
thank you for the good suggestion!
just use Nuclease-Free Water? not PBS? if not a buffer, can the exosomes be broken?
we routinely use PBS buffer. many other buffers are OK as well. Some folks use water, and looks like its fine (as there is always some residual salt etc from the isolation steps). But I prefer buffer, just in case, as stability of pure exosomes in water might be questionable
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