Is it best to just pipet gently and wait some length of time before proceeding RNA and protein isolation? I wish there was something like GlyoBlue that could be added to help with visibility.
On a related note: the protocol instructions need to be improved for clarity. The exosome isolation protocol ends with resuspending the pellet in "a convenient volume of 1x PBS or similar buffer", and indicates exosomes are then ready for downstream analysis and are stable for 1 week at 2-8oC or -20oC long-term. Seems straighforward enough and like a good place to pause and plan the next step. Except now that I'm looking more closely at the RNA and protein isolation kit, it explicity indicates pellets should be resuspended in ice-cold Exosome Resuspension Buffer if protein analysis is required, and to incubate samples 5-10 min. at RT to dissolve. (No idea why you'd want ice cold buffer if you need to dissolve at RT.) Then later it tells you if you're not immediately doing protein to store it 5-10 min. on ice or -20 long-term, or for RNA immediately to -20. If the choice of buffer and storage timing are that criticial to downstream applications, it should say so in the exosome isolation protocol rather than instruct you to do one thing only to find later you should have done it differently. The instructions are seemingly contradictory and confusing, which is very frustrating.